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  • Title: Role of one-carbon precursors in the biosynthesis of deoxyribonucleic acid in bacteriophage infected and growing cells of Escherichia coli.
    Author: NESTER EW, SPIZIZEN J.
    Journal: J Bacteriol; 1961 Dec; 82(6):867-74. PubMed ID: 14479081.
    Abstract:
    Nester, Eugene W. (Western Reserve University, Cleveland, Ohio) and John Spizizen. Role of one-carbon precursors in the biosynthesis of deoxyribonucleic acid in bacteriophage-infected and growing cells of Escherichia coli. J. Bacteriol. 82:867-874. 1961.-The ability of growing and T2 bacteriophage-infected cells of Escherichia coli to incorporate serine-3-C(14), glycine-2-C(14), formate-C(14), and formaldehyde-C(14) into purine and pyrimidine moieties of deoxyribonucleic acid (DNA) was determined. All four one-carbon precursors are effective contributors to the DNA-purines, but only glycine-2-C(14) and serine-3-C(14) are incorporated into the side chains of the pyrimidines. In addition, formate-C(14) becomes incorporated only into position 8 of the purine ring, whereas isotope from serine-3-C(14) and glycine-2-C(14) is incorporated equally into the 2 and 8 positions. No qualitative differences were observed in the patterns of incorporation of any one-carbon units in growing or bacteriophage-infected cells. However, the 3-carbon of serine serves as a more effective precursor of the 2 and 8 positions of the DNA purine ring when the cells are infected. Formate-C(14) and to a slight extent glycine-2-C(14) are somewhat better precursors of these positions when the cells are infected under appropriate conditions.
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