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  • Title: Analysis of G protein-mediated activation of phospholipase C in cultured cells.
    Author: Liu B, Wu D.
    Journal: Methods Mol Biol; 2004; 237():99-102. PubMed ID: 14501042.
    Abstract:
    Inositide-specific phospholipase C (PLC) beta can be activated by the Gq family of G alpha subunits and by the G beta gamma subunits of heterotrimeric G proteins. PLC catalyzes the hydrolysis of phosphotidylinositol 4,5-bisphosphates (PIP2) to inositol trisphosphate (IP3) and diacylglycerol (DAG). This chapter describes a cell-based assay system to determine the activity of PLC by monitoring the levels of IPs in cultured cells. This assay system can be used to examine the activation of G protein-coupled receptors (GPCRs), coupling of GPCRs to G proteins, and the interactions of G proteins to PLC beta.
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