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  • Title: R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway.
    Author: Liu Q, Rand TA, Kalidas S, Du F, Kim HE, Smith DP, Wang X.
    Journal: Science; 2003 Sep 26; 301(5641):1921-5. PubMed ID: 14512631.
    Abstract:
    The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme was purified from Drosophila S2cells and consists of two stoichiometric subunits: Dicer-2(DCR-2) and a previously unknown protein that we named R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4. Association with R2D2 does not affect the enzymatic activity of DCR-2. Rather, the DCR-2/R2D2 complex, but not DCR-2 alone, binds to siRNA and enhances sequence-specific messenger RNA degradation mediated by the RNA-initiated silencing complex (RISC). These results indicate that R2D2 bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer to RISC.
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