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  • Title: Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris.
    Author: Baumgartner P, Harper K, Raemaekers RJ, Durieux A, Gatehouse AM, Davies HV, Taylor MA.
    Journal: Biotechnol Lett; 2003 Aug; 25(15):1281-5. PubMed ID: 14514082.
    Abstract:
    The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l(-1) at the 200 1 scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.
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