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  • Title: A monoclonal antibody for distinction of invasive and noninvasive clinical isolates of Entamoeba histolytica.
    Author: Gonzalez-Ruiz A, Haque R, Rehman T, Aguirre A, Jaramillo C, Castañon G, Hall A, Guhl F, Ruiz-Palacios G, Warhurst DC.
    Journal: J Clin Microbiol; 1992 Nov; 30(11):2807-13. PubMed ID: 1452651.
    Abstract:
    Approximately 10% of the world population is infected with Entamoeba histolytica, but only 10% of the carriers develop symptomatic amebiasis. This discrepancy could be explained by the genotypic differences between the morphologically indistinguishable invasive and noninvasive strains of E. histolytica currently identified by zymodeme analysis, a technique that is unsuitable for routine diagnostic laboratories. Here we report the production of a monoclonal antibody against E. histolytica and its use in an immunofluorescence assay to identify invasive isolates cultured from stool samples of infected patients in several regions where amebiasis is endemic: Bangladesh, Colombia, and Mexico. After testing a total of 88 E. histolytica isolates, the correlation between zymodeme characterization and the immunofluorescence assay with the invasive isolate-specific monoclonal antibody was 100%. The epitope detected by the invasive isolate-specific monoclonal antibody resides in a previously undescribed internal protein with molecular masses of 84 and 81 kDa in axenic and polyxenic E. histolytica strains, respectively.
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