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  • Title: Substrate recognition and channeling of monomodules from the pikromycin polyketide synthase.
    Author: Beck BJ, Aldrich CC, Fecik RA, Reynolds KA, Sherman DH.
    Journal: J Am Chem Soc; 2003 Oct 15; 125(41):12551-7. PubMed ID: 14531700.
    Abstract:
    The unique ability of the pikromycin (Pik) polyketide synthase to generate 12- and 14-membered ring macrolactones presents an opportunity to explore the fundamental processes underlying polyketide synthesis, specifically the mechanistic details of the chain extension process. We have overexpressed and purified PikAIII (module 5) and PikAIV (module 6) and assessed the ability of these proteins to generate tri- and tetraketide lactone products using N-acetylcysteamine-activated diketides and (14)C-methylmalonyl-CoA as substrates. Comparison of the stereochemical specificities for PikAIII and PikAIV and the reported values for the DEBS modules reveals significant differences between these systems.
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