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  • Title: A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445.
    Author: Iida R, Sawazaki K, Ikeda H, Miyamoto T, Tsubota E, Takatsuka H, Masuyama M, Matsuki T, Yasuda T, Kishi K.
    Journal: J Forensic Sci; 2003 Sep; 48(5):1088-90. PubMed ID: 14535672.
    Abstract:
    We have developed a new sensitive multiplex PCR system consisting of five male-specific and polymorphic tetranucleotide STRs--DYS441 (GDB: 10013873), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 microL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had been stored for several years at room temperature.
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