These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Genosnip: SNP genotyping by MALDI-TOF MS using photocleavable oligonucleotides.
    Author: Wenzel T, Elssner T, Fahr K, Bimmler J, Richter S, Thomas I, Kostrzewa M.
    Journal: Nucleosides Nucleotides Nucleic Acids; 2003; 22(5-8):1579-81. PubMed ID: 14565470.
    Abstract:
    A photocleavable o-nitrobenzyl CE phosphoramidite building-block was synthesised and incorporated within oligonucleotides. After allele-specific primer extension, desalting was performed using genostrep purification plates. Release of the SNP information containing part through photocleavage created shortened molecules that are easily accessible for MALDI-TOF analysis. Additionally, incorporation of mass modified nucleosides enables flexible design of multiplex genotyping.
    [Abstract] [Full Text] [Related] [New Search]