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  • Title: One-step purification of E. coli elongation factor Tu.
    Author: Knudsen CR, Clark BF, Degn B, Wiborg O.
    Journal: Biochem Int; 1992 Oct; 28(2):353-62. PubMed ID: 1456956.
    Abstract:
    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one-step procedure. The construct was designed in order to make possible the release of authentic EF-Tu by cleaving the fusion protein with the protease factor Xa.
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