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  • Title: Effective bead preparation of coimmobilized methanogenic and methanotrophic bacteria for tetrachloroethene degradation.
    Author: Sung-In Y, Youn-Kyoo C, Byung-Chan L.
    Journal: Biodegradation; 2003 Oct; 14(5):347-55. PubMed ID: 14571951.
    Abstract:
    Three types of coimmobilized methanogenic and methanotrophic bacterial beads--Ca-alginate, Ba-alginate, and Ca-alginate chitosan--were used for tetrachloroethene (PCE) degradation. For the purpose of effective preparation of coimmobilized bacterial beads, the diameter and broken-loading of beads were measured. The activity tests to find the optimal bacteria concentration in the bead were performed. It was found that Ba-alginate beads had superiority in bacterial growth and the degree of strength of beads from the diameter and broken-loading tests. Also, it was shown that it is most effective to add 200 mL of methanogens into 500 mL of 2% alginate solution and 20 mL of methanotrophs into 500 mL to 2% alginate solution. When methanogens and methanotrophs were applied with the Ba-alginate bead in the actual dechlorination of PCE, the biological PCE dechlorination rate was 92%, and there was highly effective degradation of PCE based on the coimmobilized bead. Additionally, relation to the diameter (X) and broken-loading (Y) of the Ba-alginate bead was derived following equation, Y = 438.02 exp(-1.4815 X).
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