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  • Title: SecYEG proteoliposomes catalyze the Deltaphi-dependent membrane insertion of FtsQ.
    Author: van der Laan M, Nouwen N, Driessen AJ.
    Journal: J Biol Chem; 2004 Jan 16; 279(3):1659-64. PubMed ID: 14578344.
    Abstract:
    In Escherichia coli, the insertion of most inner membrane proteins is mediated by the Sec translocase. Ribosome-bound nascent chains of Sec-dependent inner membrane proteins are targeted to the SecYEG complex via the signal recognition particle pathway. We now demonstrate that the signal recognition particle-dependent co-translational membrane targeting and membrane insertion of FtsQ can be reconstituted with proteoliposomes containing purified SecYEG. SecA and a transmembrane electrical potential are essential for the translocation of the large periplasmic domain of FtsQ, whereas co-reconstituted YidC has an inhibitory effect. These data demonstrate that membrane protein insertion can be reconstituted with a minimal set of purified Sec components.
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