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  • Title: Determination of the volatile chemical constituents of Notoptergium incium by gas chromatography-mass spectrometry and iterative or non-iterative chemometrics resolution methods.
    Author: Guo FQ, Liang YZ, Xu CJ, Huang LF.
    Journal: J Chromatogr A; 2003 Oct 17; 1016(1):99-110. PubMed ID: 14601831.
    Abstract:
    The qualitative and quantitative determination of the chemical constitutes in traditional Chinese medicine (TCM) is an important task, which builds the foundation of the theory of pharmacological activity. The hyphenated chromatography instruments combined with the related chemometric methods provide powerful tools for the resolution of such complex systems. The familiar chemometrics methods can be roughly divided into two different kinds, the iterative one such as orthogonal projection approach (OPA) and non-iterative one representing by evolving window orthogonal projection (EWOP). One can use different kinds of methods according to overlapping condition, and then the measured data matrix can be resolved into pure concentration profiles and mass spectra of the chemical components with relative high efficiency and acceptable accuracy. One kind of TCM, named Notoptergium incium (NI) was analyzed by gas chromatography-mass spectrometry (GC-MS) and resolved by above chemometric approach. Experiment results show the efficiency and convenience of the proposed approach. 65 of the 98 separated constituents in essential oil, accounting for 92.13%, were identified by mass spectroscopy (MS).
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