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  • Title: Use of dried whole blood spots to measure CD4+ lymphocyte counts in HIV-1-infected patients.
    Author: Mwaba P, Cassol S, Pilon R, Chintu C, Janes M, Nunn A, Zumla A.
    Journal: Lancet; 2003 Nov 01; 362(9394):1459-60. PubMed ID: 14602443.
    Abstract:
    As antiretroviral drugs become widely available in developing countries, practical, field-friendly, and cheap methods of measuring CD4+ lymphocyte counts need to be developed. We tested use of whole blood spots dried on filter paper to measure CD4+ lymphocyte counts. We obtained blood from 42 HIV-1-infected patients from Zambia. We dried blood spots on filter paper and measured CD4+ lymphocyte counts with an established commercial enzyme immunoassay. We compared these measurements with those obtained from matched liquid whole-blood samples analysed with standard flow cytometry. Results of the filter-paper method accorded well with flow cytometry CD4 counts greater than 200 cells/microL (mean difference 13.6 [SD 52.4]). Dried whole blood stored on filter paper could be developed into a field-friendly alternative for CD4+ lymphocyte count measurements.
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