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  • Title: Preliminary comparison of three LightCycler PCR assays for the detection of herpes simplex virus in swab specimens.
    Author: Whiley DM, Syrmis MW, Mackay IM, Sloots TP.
    Journal: Eur J Clin Microbiol Infect Dis; 2003 Dec; 22(12):764-7. PubMed ID: 14605937.
    Abstract:
    Three Herpes Simplex Virus LightCycler polymerase chain reaction assays were compared for the detection of herpes simplex virus in 48 swab specimens. The assays comprised of one in-house assay and two commercial kits: the Artus HSV LC RealArt PCR kit and the Roche LightCycler HSV 1/2 Detection kit. On the whole, the three assays had comparable sensitivities. However, differentiation of herpes simplex virus types 1 and 2 by melting curve analysis was problematic in all assays. Overall, the results highlight the limitations of typing herpes simplex virus by melting curve analysis.
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