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  • Title: The retention behaviour of conjugated bile acids in reversed phase high performance liquid chromatography.
    Author: Zhang YK, Chen N, Zao RH, Li T, Wang ZY, Lu PC.
    Journal: Biomed Chromatogr; 1992; 6(5):255-7. PubMed ID: 1463939.
    Abstract:
    The retention behaviour of conjugated bile acids has been studied in a reversed phase high performance liquid chromatographic (RP-HPLC) system by using the mixture of methanol and aqueous phosphate buffer as the mobile phase. The retentions of the conjugates in RP-HPLC have been found to be mainly controlled by the glycine and taurine groups. The selectivity between five different glycine and taurine conjugated bile acids is a constant in RP-HPLC. This selectivity has been used for peak identification in the practical separation of conjugated bile acids.
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