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  • Title: Effects of serum deprivation on myofibrillar proteolysis in chick myotube cultures.
    Author: Nakashima K, Nonaka I, Masaki S.
    Journal: Biosci Biotechnol Biochem; 2003 Nov; 67(11):2455-8. PubMed ID: 14646208.
    Abstract:
    We examined the effects of serum deprivation on myofibrillar proteolysis in chick myotubes. Myotubes were incubated with serum-free medium for 24 hours. N(tau)-methylhistidine release, as an index of myofibrillar proteolysis, as well as protease activities such as calpain, proteasome, and cathepsins (B+L and D) activities were increased by serum deprivation. These results indicate that serum deprivation induces calpain, proteasome, and cathepsins activities, resulting in an increase in myofibrillar proteolysis in chick myotubes.
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