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  • Title: Accelerating enzymatic hydrolysis of chitin by microwave pretreatment.
    Author: Roy I, Mondal K, Gupta MN.
    Journal: Biotechnol Prog; 2003; 19(6):1648-53. PubMed ID: 14656136.
    Abstract:
    Response surface analysis was used to determine optimum conditions [2% (w/v) chitin, 57.5 degrees C, 38 min] for microwave irradiation of chitin to improve its enzymatic hydrolysis. V(max)/K(m) of cabbage chitinase toward untreated and microwave-irradiated chitin was found to be 21.1 and 31.7 nmol h(-1) mg(-2) mL, respectively. Similar improvement was observed in the case of pectinase in its unusual catalytic activity of chitin degradation. It was found that a greater extent of chitin hydrolysis by chitinase was possible after the substrate chitin was irradiated with microwaves.
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