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  • Title: [Identification of Mycobacterium tuberculosis strains and a simultaneous identification of their drug resistance by the hybridization method on oligonucleotide microchips].
    Author: Griadunov DA, Mikhaĭlovich VM, Lapa SA, Rudinskiĭ NI, Barskiĭ VE, Chudinov AV, Zasedatelev AS, Mirzabekov AD.
    Journal: Mol Gen Mikrobiol Virusol; 2003; (4):24-7. PubMed ID: 14664159.
    Abstract:
    A method of multiplex polymerase chain reaction (PCR) with subsequent hyoridization on oligonucleotide microchips was worked out to identify the Mycobacterium tuberculosis complex and to determine simultaneously the bacterial sensitivity to 2 first-line drugs, i.e. rifampin and isoniazid. The method provides for detecting above 95% of rifampin-resistant and around 80% of isoniazid-resistant strains within 1 day.
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