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  • Title: Development of a sheathless interface between reversed-phase capillary HPLC and ICPMS via a microflow total consumption nebulizer for selenopeptide mapping.
    Author: Schaumlöffel D, Ruiz Encinar J, Łobiński R.
    Journal: Anal Chem; 2003 Dec 15; 75(24):6837-42. PubMed ID: 14670043.
    Abstract:
    A sheathless interface based on a total consumption micronebulizer operating at flow rates in the range 0.5-7.5 microL min(-1) was developed between capillary HPLC and ICPMS. It allowed the efficient nebulization and transport into the plasma of mobile phases containing up to 100% organic solvent without either cooling the spray chamber or oxygen addition. The coupled system was applied to selenopeptide mapping in a protein fraction isolated from a selenized yeast extract. The detection limits were 150 (80Se) and 200 fg (82Se) for a quadrupole instrument with and without a collision cell, respectively, which is a factor 100-150 less than that reported elsewhere for HPLC-ICPMS. The minimal peak broadening ( approximately 5 s at the half-height) allowed baseline resolution of a mixture containing more than 30 selenopeptides, many of which could not be separated using the conventional HPLC-ICPMS coupling.
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