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Title: Purification and properties of adenosine triphosphatase solubilized from beef heart mitochondria by chloroform. Author: Kopecký J, Houstĕk J, Drahota Z. Journal: Mol Cell Biochem; 1977 Dec 29; 18(2-3):77-80. PubMed ID: 146824. Abstract: Soluble, oligomycin-insensitive ATPase released from beef heart mitchondria by chloroform extraction can be further purified by Sepharose 6B gel filtration. This purification increases enzyme activity 4--5 times (100-130U/mg). According to specific activity, high purity and ability to reconstitute oligomycin-sensitive complex, isolated ATPase is quite comparable with enzyme preparations isolated by other methods.[Abstract] [Full Text] [Related] [New Search]