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  • Title: Decreased expression of equine herpesvirus-1 early and late genes in the placenta of naturally aborted equine fetuses.
    Author: Kimura T, Hasebe R, Mukaiya R, Ochiai K, Wada R, Umemura T.
    Journal: J Comp Pathol; 2004 Jan; 130(1):41-7. PubMed ID: 14693123.
    Abstract:
    Intrauterine infection with equine herpesvirus-1 (EHV-1) has been considered to be the consequence of transplacental transmission of the virus following maternal cell-associated viraemia. In this study the state of EHV-1 gene expression in the placenta of seven naturally aborted equine fetuses was examined. Neither lesions nor viral antigens were detected in the placenta of the fetuses. The amount of infectious virus in the placentas was considerably lower than that in the fetal lungs, which showed pneumonia and typical herpesvirus inclusions. Quantitative dot blot hybridization with probes specific for immediate-early (IE), early (ICP0), and late (gD and gK) genes revealed that the placentas expressed the IE gene at a level comparable with that in the lungs; however, expression of the ICP0, gD and gK genes was significantly weaker in the placentas than in the lungs. In-situ hybridization demonstrated that both IE and gK RNAs were distributed mainly in the cytoplasm of trophoblasts. These results suggest that the low level of early and late gene transcription may be related to the limited production of viral progeny and the lack of immunoreactivity for viral antigen in trophoblasts infected with EHV-1.
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