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  • Title: [Study on drug-resistance of Mycobacterium tuberculosis isolated from coal workers with silico-tuberculosis].
    Author: Jiang P, Li H, Chen D, Wang W, Feng B, Wang Z, Wang G, Li S, Han H.
    Journal: Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi; 2002 Apr; 20(2):100-2. PubMed ID: 14694621.
    Abstract:
    OBJECTIVE: To study the gene mutation and streptomycin, isoniazid or rifampicin resistance of Mycobacterium isolated from silico-tuberculosis patient's sputum so as to find a more effective therapy for this disease. METHODS: Mycobacteria tuberculosis were separated from 96 coal worker with silico-tuberculosis firstly. Then rpsL, KatG and rpoB fragments of genome were copied with PCR and compared their SSCP profiles with standard strains. RESULTS: 67 strains of streptomycin, isoniazid or rifampicin resistant Mycobacteria tuberculosis were found in routine drug resistance test, with the percentages of 80.5% (54/67), 58.2% (39/67) respectively. PCR-SSCP showed that out of 67 drug-resistant strains, 66(98.5%) of rpsL, 47(70.1%) of rpoB and 42(62.7%) of KatG appeared abnormal. CONCLUSION: Most of the resistant strains appeared gene mutation. The mution rates were higher than the results from routine drug resistance test.
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