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  • Title: A (4R)- or a (4S)-fluoroproline residue in position Xaa of the (Xaa-Yaa-Gly) collagen repeat severely affects triple-helix formation.
    Author: Barth D, Milbradt AG, Renner C, Moroder L.
    Journal: Chembiochem; 2004 Jan 03; 5(1):79-86. PubMed ID: 14695516.
    Abstract:
    The triple-helical fold of collagen requires the presence of a glycine residue at every third position in the peptide sequence and is stabilized by proline and (4R)-4-hydroxyproline residues in positions Xaa and Yaa of the (Xaa-Yaa-Gly) triplets, respectively. Regular down/up puckering of these Xaa/Yaa residues is possibly responsible for the tight packing of the three peptide strands, which have a polyproline-II-like structure, into the supercoiled helix. (4R)-Configured electronegative substituents such as a hydroxy group or a fluorine substituent on the pyrrolidine ring of the residue in the Yaa position favor the up pucker and thus significantly stabilize the triple helix. A similar effect was expected from the corresponding (4S)-isomers in the Xaa positions, but the opposite effect has been observed with (4S)-hydroxyproline, a result that has been speculatively attributed to steric effects. In this study, (4R)- and (4S)-fluoroproline residues were introduced into the Xaa position and potential steric effects were thus avoided. Contrary to expectations, (4S)-fluoroproline prevents triple-helix formation, whereas (4R)-fluoroproline stabilizes the polyPro II conformation, but without supercoiling of the three strands. The latter observation suggests that folding of the single chains into a polyproline II helix is not directly associated with triple helix formation and that fine tuning of van der Waals contacts, electrostatic interactions, and stereoelectronic effects is required for optimal packing into a triple helix.
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