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  • Title: A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor.
    Author: Garden SR, Doellgast GJ, Killham KS, Strachan NJ.
    Journal: Biosens Bioelectron; 2004 Feb 15; 19(7):737-40. PubMed ID: 14709392.
    Abstract:
    A fibre optic evanescent wave sensor is used for the rapid detection of thrombin. Coagulation of solution phase fluorescently labelled fibrinogen to unlabelled fibrinogen bound to the surface of the fibre optic is observed in real time by the evanescent wave sensor. Thrombin concentrations down to 0.01 NIHml(-1) are detectable within 5 min. The potential application of this technique for rapid amplified immunosensing is discussed.
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