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Title: [Identification of catalytically active groups in inulinase from Bacillus polymyxa 722]. Author: Zherebtsov NA, Abramova IN, Shelamova SA, Popova TN. Journal: Prikl Biokhim Mikrobiol; 2003; 39(6):619-24. PubMed ID: 14714473. Abstract: Inulinase from Bacillus polymyxa 722 hydrolyzing a polyfructosan inulin was studied. The dependence of inulinase activity on pH, measurements of pK value, calculation of ionization heat, photoinactivation with methylene blue, and inhibition with p-chloromercuribenzoate suggest that the active center of this enzyme contains imidazole and sulfhydryl groups. A possible mechanism underlying cleavage of beta-2,1-fructoside bonds in the inulin molecule with inulinase is considered.[Abstract] [Full Text] [Related] [New Search]