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  • Title: Abscisic acid studied by fluorescent techniques.
    Author: del Castillo B, Alvarez-Builla J, Martinez-Honduvilla CJ.
    Journal: Ital J Biochem; 1977; 26(6):437-43. PubMed ID: 147250.
    Abstract:
    Crude analytical abscisic acid (ABA) exhibited fluorescence (lambdaex 340, lambdaem 425 nm). The results demonstrated a relationship between fluorescence response and ABA concentration, in the range of 0.04-0.38 microgram/ml. Chromatographic purification increased the emission signal, and oxidation induced by adding a sulphuric acid: ethanol (1:1) mixture also increased and displaced it (lambdaex 450, lambdaem 495 nm). Using these properties, a fluorimetric method for identification and evaluation of ABA, had been developed, and used for inhibitor recognition in P. pinea seed coats.
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