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  • Title: [Protein and mRNA expression of nitric oxide synthase isoforms I and III in rat penile spongium tissues].
    Author: Shi JP, Zhao YM, Qi G.
    Journal: Zhonghua Nan Ke Xue; 2003 Dec; 9(9):663-5, 668. PubMed ID: 14727352.
    Abstract:
    OBJECTIVE: To investigate protein and mRNA expression of nitric oxide synthase (NOS) isoforms I and III in rat penile tissues. METHODS: Penile tissues from male SD rats were investigated. The protein expression of NOS I and III in rat penis was confirmed by immunoprecipitation and Western blot. Gene expression of both NOS I and III was identified by reverse transcription-polymerase chain reaction. RESULTS: The mRNA and protein products of NOS I and III are both expressed in normal rat penile spongium tissues. But, NOS III mRNA and protein products are more highly expressed than those of NOS. CONCLUSIONS: These findings support the possibility that penile erection is regulated by different NOS isoforms in rat penile tissues.
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