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Title: Coenzyme specificity of enzymes in the oxidative pentose phosphate pathway of Gluconobacter oxydans. Author: Tonouchi N, Sugiyama M, Yokozeki K. Journal: Biosci Biotechnol Biochem; 2003 Dec; 67(12):2648-51. PubMed ID: 14730146. Abstract: The coenzyme specificity of enzymes in the oxidative pentose phosphate pathway of Gluconobacter oxydans was investigated. By investigation of the activities of glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) in the soluble fraction of G. oxydans, and cloning and expression of genes in Escherichia coli, it was found that both G6PDH and 6PGDH have NAD/NADP dual coenzyme specificities. It was suggested that the pentose phosphate pathway is responsible for NADH regeneration in G. oxydans.[Abstract] [Full Text] [Related] [New Search]