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  • Title: [The study of hBMP-4 gene modified tissue-engineered bone].
    Author: Jiang XQ, Zhang ZY, Chen JG, Ma GX, Chen CJ.
    Journal: Zhonghua Kou Qiang Yi Xue Za Zhi; 2003 Sep; 38(5):390-2. PubMed ID: 14746330.
    Abstract:
    OBJECTIVE: Bone marrow stromal cells (bMSCs) of rabbits transferred with mammalian hBMP-4 expression plasmid were used to construct tissue-engineered bone. Gene therapy combined with tissue-engineering technique was explored to further improve osteogenesis. METHODS: pEGFP-hBMP-4 plasmid was constructed by subcloning technique. bMSCs were then transferred with either pEGFP-hBMP-4, pEGFP plasmid by lipofectamine or left uninfected in vitro. The cells from the 3 groups were combined with natural non-organic bone (NNB) to construct tissue-engineered bones, which were subcutaneously implanted into nude mice (6 implants per group) for 4 weeks. Specimens were evaluated through histological and computerized new bone formation analysis. RESULTS: pEGFP-hBMP-4 plasmid was successfully constructed. bMSCs could attach and proliferate on the surface on NNB. In vivo experiment showed that new bone formation in pEGFP-hBMP-4 group was higher than those of the control groups. CONCLUSIONS: Tissue-engineered bone using hBMP-4 gene modified bMSCs might be an ideal alternative for the repair of bone.
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