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  • Title: Expression, crystallization and preliminary X-ray studies of the recombinant PTB domain of mouse dok1 protein.
    Author: Shi N, Liu Y, Ni M, Yang M, Wu J, Peng Y, Gao F, Sun F, Peng X, Qiang B, Rao Z, Yuan J.
    Journal: Acta Crystallogr D Biol Crystallogr; 2004 Feb; 60(Pt 2):334-6. PubMed ID: 14747716.
    Abstract:
    The PTB domain of mouse dok1 fusion protein has been overexpressed in Escherichia coli and crystallized in a form suitable for X-ray crystallographic study. Crystals have been obtained using the vapour-diffusion method and belong to space group P2(1)2(1)2(1). X-ray diffraction data were collected in-house to 2.5 A resolution. A selenomethionine (SeMet) dok1 PTB fusion-protein derivative was expressed using the same expression system, purified in a reductive environment and crystals were obtained under similar conditions. Subsequently, three different wavelength data sets from the derivative crystal were collected to 2.5 A resolution at SPring-8.
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