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  • Title: Structural similarity of E. coli 5S rRNA in solution and within the ribosome.
    Author: Skibinska L, Banachowicz E, Gapiński J, Patkowski A, Barciszewski J.
    Journal: Biopolymers; 2004 Feb 15; 73(3):316-25. PubMed ID: 14755567.
    Abstract:
    The article presents translational and rotational diffusion coefficients of 5S rRNA determined experimentally by the method of dynamic light scattering (DLS) and its comparison with the values predicted for different models of this molecule. The tertiary structure of free 5S rRNA was proposed on the basis of the atomic structures of the 5S rRNA from E. coli and H. marismortui extracted from the ribosome. A comparison of the values of DT, tauR, and Rg predicted for different models with experimental results for the free molecule in solution suggests that free 5S rRNA is less compact than that in the complex with ribosomal proteins. In general, the molecules of 5S rRNA consist of three domains: a short one and two longer ones. As follows from a comparison of the results of our simulations with experimental values, in the molecule in solution the two closest helical fragments of the longer domains remain collinear, whereas the short domain takes a position significantly deviated from them.
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