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  • Title: [A study on the mutation of P gene region including YMDD motif in hepatitis B virus].
    Author: Feng B, Wei L, Chen M, Li XH.
    Journal: Zhonghua Gan Zang Bing Za Zhi; 2004 Jan; 12(1):29-31. PubMed ID: 14761279.
    Abstract:
    OBJECTIVE: To investigate the primary structure and heterogenenity of P gene region including YMDD motif in hepatitis B virus. METHODS: From serum samples collected from 4 patients who had never been treated with anti-viral drugs, DNA fragments of 1057bp long of P gene were amplified and cloned into pUC19. Twenty positive clones were chosen randomly from each sample. The YMDD motif mutation was detected by mismatched PCR-RFLP. Finally last ten positive clones of two samples were sequenced. RESULTS: Nucleotide mutation rates among clones of Sample 1 and 2 were 0.3% - 1.1%, 0.4% - 1.7%, respectively. Among 80 clones, the variations from YMDD to YMGD were revealed in two clones. CONCLUSION: There are HBV quasispecies in the P gene region including YMDD motif of hepatitis B virus and a novel mutation of YMDD motif in the sera of patients without being therapied by anti-viral drugs.
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