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  • Title: [Effect of cyclin G1 antisense oligodeoxynucleotides (ASON) on the growth of HL-60 cells in nude mice].
    Author: Jia JS, Xu SR, Jia CR, Ma J, Ha S, Yao YR, Wang Y, Shi CY.
    Journal: Zhonghua Xue Ye Xue Za Zhi; 2003 Dec; 24(12):648-51. PubMed ID: 14761614.
    Abstract:
    OBJECTIVE: To study the inhibition effect of cyclin G(1) antisense oligodeoxynucleotides (ASON) on the growth of HL-60 cells in nude mice. METHODS: (1) Nude mice were divided into control group, sense oligodeoxynucleotides (SON) group and ASON group. After (60)Co radiation, with HL-60 cells SON group and ASON group were subcutaneously innoculated; (2) The weight and volume of tumors were continually measured; (3) The morphology of tumor cells was observed by microscope; (4) The protein and mRNA expression levels of cyclin G(1) were determined by flow cytometry (FCM) and reverse transcription polymerase chain reaction (RT-PCR); (5) The cell apoptosis was detected by electron microscopy and FCM. RESULTS: (1) The inhibition rate of tumor in ASON group was 69.4%. In ASON group, the wight and volume of tumor were significantly lower than those in SON group and control group. (2) The HL-60 cells in ASON group showed morphologically smaller nuclei, less mitosis, less heteromorphosis and apoptosis. CONCLUSION: The cyclin G(1) ASON can inhibit the growth of HL-60 cells in nude mice and induce apoptosis.
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