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  • Title: Application of preparative high-speed counter-current chromatography for separation of chlorogenic acid from Flos Lonicerae.
    Author: Lu HT, Jiang Y, Chen F.
    Journal: J Chromatogr A; 2004 Feb 13; 1026(1-2):185-90. PubMed ID: 14763745.
    Abstract:
    Chlorogenic acid, an ester formed between caffeic acid and quinic acid, is a major phenolic compound in the traditional Chinese medicinal herb Flos Lonicerae. The separation and purification of chlorogenic acid from the crude extract of Flos Lonicerae was achieved by high-speed counter-current chromatography (HSCCC). A high acid, highly polar two-phase solvent system containing n-butanol-acetic acid-water (4:1:5) was run on a preparative scale. The upper phase was used as the mobile phase in the head to tail elution mode. A 300-mg quantity of the crude extract containing 5.97% chlorogenic acid was loaded on a 342-ml HSCCC column. Double separations were performed with the same solvent system yielding 16.9 mg chlorogenic acid at 94.8% purity with approximately 90% recovery.
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