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  • Title: Effect of overexpression of Actinobacillus succinogenes phosphoenolpyruvate carboxykinase on succinate production in Escherichia coli.
    Author: Kim P, Laivenieks M, Vieille C, Zeikus JG.
    Journal: Appl Environ Microbiol; 2004 Feb; 70(2):1238-41. PubMed ID: 14766613.
    Abstract:
    Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc::kan, PEPCK overexpression increased succinate production 6.5-fold.
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