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  • Title: Evaluation of Syva's enzyme immunoassay for the detection of Chlamydia trachomatis in urogenital specimens.
    Author: Moncada J, Schachter J, Bolan G, Nathan J, Shafer MA, Clark A, Schwebke J, Stamm W, Mroczkowski T, Seliborska Z.
    Journal: Diagn Microbiol Infect Dis; 1992; 15(8):663-8. PubMed ID: 1478048.
    Abstract:
    A newly developed microwell enzyme immunosorbent assay (EIA) system by Syva Company (Palo Alto, CA) can detect Chlamydia trachomatis in < 3 hr. It uses a polyclonal antibody to chlamydial lipopolysaccharide and end points are determined with a spectrophotometer. Three clinical trial sites (University of California Medical Center, San Francisco, CA; University of Washington, Seattle, WA; and Louisiana State University Medical Center, New Orleans, LA), compared this EIA with tissue culture (TC) for identifying Chlamydia in urogenital specimens. Overall prevalence by TC was 10.4% (136 of 1306). When tests were compared with TC (using vials or microtiter plates and a fluorescent antibody stain), we found an EIA sensitivity of 93.4% (127 of 136) and a specificity of 98.1% (1148 of 1170). This EIA has a performance profile that is, at the very least, comparable with other nonculture methods for diagnosing genital tract infections with C. trachomatis.
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