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  • Title: Isolation and structures of microcystins from a cyanobacterial water bloom (Finland).
    Author: Namikoshi M, Sivonen K, Evans WR, Sun F, Carmichael WW, Rinehart KL.
    Journal: Toxicon; 1992 Nov; 30(11):1473-9. PubMed ID: 1485342.
    Abstract:
    A hepatotoxic cyanobacterial (blue-green algal) water bloom was collected from a constructed water reservoir in Finland. The water bloom contained two cyanobacterial species, Microcystis aeruginosa and Aphanizomenon flos-aquae. Two hepatotoxins, 1 and 2, were isolated from extracts of lyophilized cells. The structures of 1 and 2 were assigned based upon their amino acid analyses on a Waters Pico Tag HPLC system and a chiral GC capillary column (Chirasil Val III), fast atom bombardment mass spectrometry (FABMS), high resolution FABMS, and tandem FABMS data. Toxin 1 was identical to a previously reported compound, [D-Asp3]microcystin-RR. Toxin 2 was new and was assigned the structure [D-Asp3]microcystin-YR.
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