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  • Title: Characterization of immune complex components by dot blot analysis.
    Author: Hoffman WL, Kelly PJ, Ruggles AO.
    Journal: Anal Biochem; 1992 Nov 15; 207(1):44-50. PubMed ID: 1489098.
    Abstract:
    A method is described for the characterization of immune complex components by dot blot analysis. After isolation by chromatographic techniques and precipitation with polyethylene glycol, immune complexes were dissociated in 0.1 M phosphate (pH 2) and bound to a nitrocellulose membrane in a dot blot unit. Biotinylated probes were then used to identify the following immune complex components: specific antigens, biologically active antibodies, antibody isotypes, antibody subclasses, antibody idiotypes, and rheumatoid factors. This nonradioactive procedure takes less than 2 h to perform and has been used to analyze immune complexes isolated from sera (rabbit and human) and synovial fluid (human).
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