These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Differential responses of neocortical neurons to glucose and/or O2 deprivation in the human and rat.
    Author: Jiang C, Haddad GG.
    Journal: J Neurophysiol; 1992 Dec; 68(6):2165-73. PubMed ID: 1491265.
    Abstract:
    1. Intracellular recordings were performed in human and rat neocortical neurons with in vitro brain slice techniques. Baseline cellular properties and the effect of O2 and glucose deprivation on these neurons were studied. 2. Intracellular labelings of electrophysiologically identified neurons showed that most neurons recorded from layers 4 and 5 of the neocortex in both rats and humans were pyramidal cells with a regular-spiking or a burst firing pattern. 3. A period of complete anoxia (4-5 min) induced little or no change in membrane potential (Vm) in rat and human neocortical neurons, contrasting with the major depolarization we have previously observed in rat brainstem neurons during a similar period of anoxia. Evident depolarization occurred only when the slices were exposed to a more prolonged period of anoxia (> 7 min in rats and > 10 min in humans). 4. Membrane input resistance (Rm) of neocortical neurons decreased in both species during anoxia. In human neocortical neurons, Rm decreased by a mean of 22% with a marked increase in rheobase and suppression in spontaneous excitatory postsynaptic potentials (EPSPs). Interestingly, the increase in rheobase in human cells occurred even at an early stage (post 2-3 min in anoxia), when Vm and Rm had not yet changed. 5. Perfusing slices with a glucose-free medium for 1-2 h produced a relatively modest change in Vm (mean congruent to 28 mV). However, combined deprivation of both glucose and O2 resulted in a major depolarization (mean congruent to 50 mV) within 5-10 min in both human and rat neocortical neurons.(ABSTRACT TRUNCATED AT 250 WORDS)
    [Abstract] [Full Text] [Related] [New Search]