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Title: [Brief counting method of airborne Cryptomeria japonica pollen by a combination of fluorescence antibody staining and flow cytometry]. Author: Takahashi Y, Yoshimura S, Masago H, Inouye K, Shirai T, Nagoya T, Sakaguchi M, Inouye S, Katagiri S. Journal: Arerugi; 1992 Nov; 41(11):1611-3. PubMed ID: 1492796. Abstract: Airborne pollens collected in a pollen collector (Virtual Impactor) was treated with a fluorescein isothiocyanate-labeled monoclonal antibody (KW-S10) which was strictly specific to Japanese cedar pollen antigen (Cry j I). Flow cytometric analysis revealed that the intensity of fluorescence of the pollen samples treated with the antibody was greater than that of non-treated reference pollen or the antibody treated Hinoki-cypress pollen. By use of this method, it may be possible to display the airborne pollen concentration within 20 min after sampling.[Abstract] [Full Text] [Related] [New Search]