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  • Title: Assays for measuring in vitro basophil activation induced by recombinant allergens.
    Author: Valent P, Hauswirth AW, Natter S, Sperr WR, Bühring HJ, Valenta R.
    Journal: Methods; 2004 Mar; 32(3):265-70. PubMed ID: 14962761.
    Abstract:
    The diagnosis of type I allergy is essentially based on clinical data, skin tests, and measurements of allergen-specific IgE. However, the determination of specific IgE per se does not permit a definitive conclusion concerning the response of effector cells to the respective allergen(s) and consecutive clinical symptoms in all patients. In an attempt to overcome this problem, a number of basophil-activation assays have been developed during the last few years. Today, allergen-induced activation of blood basophils can be employed as a specific and reliable measure of IgE-dependent responses in sensitized individuals. Using recombinant allergens and basophil-specific markers, these novel assays appear to serve as simple and useful tests in component-resolved diagnosis of type I allergies. In the current article, the biochemical, functional, and technical background of these basophil tests is discussed.
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