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  • Title: A novel method for "Wet" SEM.
    Author: Barshack I, Kopolovic J, Chowers Y, Gileadi O, Vainshtein A, Zik O, Behar V.
    Journal: Ultrastruct Pathol; 2004; 28(1):29-31. PubMed ID: 14967596.
    Abstract:
    Progress in the processing of wet tissues, without the need of fixation and complex preparation procedures, may facilitate the microscopic examination of tissues and cells. Microscopic examination of tissues is a central tool in clinical diagnosis as well as in diverse areas of research. The authors present the application of Wet SEM, a technology for imaging fully hydrated samples at atmospheric pressure in a scanning electron microscope (SEM). The technique is based on 2 principles. First, samples are imaged in sealed specimen capsules and are separated from the evacuated interior of the electron microscope by a thin, electron-transparent partition membrane that is strong enough to sustain a 1-atm pressure difference. Second, imaging is done in a SEM, based on detection of backscattered electrons, which penetrate a few microns into the specimen and thus give information on the cellular level.
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