These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [The determination of free and bound methylated nucleobases in food].
    Author: Jänicke S, Montag A.
    Journal: Z Ernahrungswiss; 1992 Jun; 31(2):91-102. PubMed ID: 1496834.
    Abstract:
    The following method was developed for the qualitative and quantitative determination of methylated purine and pyrimidine bases in food; some RNA-rich food was also investigated. The bases were liberated from nucleic acids, nucleotides, and nucleosides by acid hydrolysis with trifluoroacetic and formic acid (1/1, V/V) at 240 degrees C in a pressure-digestion system. Purification and fractionation of hydrolysates were achieved by sorbent extraction with an RP 18 column and a cation exchange column. The major and methylated nucleobases were determined by ion-exchange chromatography and reversed-phase-HPLC.
    [Abstract] [Full Text] [Related] [New Search]