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  • Title: Maintenance of meiotic prophase arrest in vertebrate oocytes by a Gs protein-mediated pathway.
    Author: Kalinowski RR, Berlot CH, Jones TL, Ross LF, Jaffe LA, Mehlmann LM.
    Journal: Dev Biol; 2004 Mar 01; 267(1):1-13. PubMed ID: 14975713.
    Abstract:
    Maintenance of meiotic prophase arrest in fully grown vertebrate oocytes depends on an elevated level of cAMP in the oocyte. To investigate how the cAMP level is regulated, we examined whether the activity of an oocyte G protein of the family that stimulates adenylyl cyclase, Gs, is required to maintain meiotic arrest. Microinjection of a dominant negative form of Gs into Xenopus and mouse oocytes, or microinjection of an antibody that inhibits the Gs G protein into zebrafish oocytes, caused meiosis to resume. Together with previous studies, these results support the conclusion that Gs-regulated generation of cAMP by the oocyte is a common mechanism for maintaining meiotic prophase arrest in vertebrate oocytes.
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