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  • Title: Direct monitoring of enzymatic glucan hydrolysis on a 27-MHz quartz-crystal microbalance.
    Author: Nishino H, Nihira T, Mori T, Okahata Y.
    Journal: J Am Chem Soc; 2004 Mar 03; 126(8):2264-5. PubMed ID: 14982404.
    Abstract:
    By using a highly sensitive 27-MHz glucan-immobilized quartz-crystal microbalance, we could follow kinetically all processes (enzyme binding and release, kon and koff, and intramolecular hydrolysis rates, kcat) of glucan hydrolysis by glucoamylase by detecting directly the formation and decay of the enzyme-substrate complex as mass changes.
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