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  • Title: Flow cytometry in the diagnosis of lymphoproliferative lesions of the orbit and eye adnexa in fine needle aspiration biopsy.
    Author: Wolska-Szmidt E, Masiuk M, Krzystolik K, Chosia M.
    Journal: Pol J Pathol; 2003; 54(4):253-9. PubMed ID: 14998294.
    Abstract:
    The goal of the investigation was to evaluate the validity of routine cytology and flow cytometry in the differential diagnosis of lymphoproliferative disorders of the orbit and eye adnexa. The investigations were carried out on materials originating from fine needle aspiration biopsy performed in 14 patients, including 9 females and 5 males aged 31-81 years. Apart from routine cytology, cytometric studies were also performed. Based on cytology, non-Hodgkin's lymphomas were diagnosed in six patients, while one was suspected of NHL. In seven patients the diagnosis was ambivalent, since based on cytology it was impossible to conclusively determine the biological character of the lesion, i.e. state whether it was benign or malignant. Flow cytometry was performed in 14 patients, but ultimately the results were available in 12 individuals, since in two cases the material was scant enough to exclude any assessment. Thanks to using a panel of monoclonal antibodies against light chains kappa and lambda, as well as against CD surface antigens, the authors demonstrated clonality in 90% (9/10) of NHL cases; of this number, in 7 instances the test confirmed the preliminary diagnosis and in 2 cases rendered the diagnosis more precise. On the other hand, in 3 cases no clonality was noted; of this number, in two instances the diagnosis was specified as a benign lesion (BLPL) and in one case the assessment of clonality had no impact on the final diagnosis. Out of 12 investigated aspirates, in 11 cases the result concerning clonality affected the final diagnosis. The evaluation of cellular phenotype in flow cytometry in materials obtained in the course of FNAB is a fast and sensitive method and in many cases allows for avoiding a surgical biopsy.
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