These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The mechanism of myosin VI translocation and its load-induced anchoring.
    Author: Altman D, Sweeney HL, Spudich JA.
    Journal: Cell; 2004 Mar 05; 116(5):737-49. PubMed ID: 15006355.
    Abstract:
    Myosin VI is thought to function as both a transporter and an anchor. While in vitro studies suggest possible mechanisms for processive stepping, a biochemical basis for anchoring has not been demonstrated. Using optical trapping, we observed myosin VI stepping against applied forces. Step size is not strongly affected by such loads. At saturating ATP, myosin VI kinetics shows little dependence on load until, at forces near stall, its stepping slows dramatically as load increases. At subsaturating ATP or in the presence of ADP, stepping kinetics is significantly inhibited by load. From our results, we propose a mechanism of myosin VI stepping that predicts a regulation through load of the motor's roles as transporter and anchor.
    [Abstract] [Full Text] [Related] [New Search]