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  • Title: [Determination of schisandrin in shengmaiyin by HPLC].
    Author: Wu XF, Chen H, Zhao ZR.
    Journal: Zhongguo Zhong Yao Za Zhi; 2003 Jan; 28(1):39-40, 72. PubMed ID: 15015264.
    Abstract:
    OBJECTIVE: To determine the concentration of schisandrin in Shengmaiyin with HPLC. METHOD: The sample was extracted with ethyl acetate through supersonic wave. The solution was filtrated and evaporated. The residue was resolved with methanol and determined by HPLC using PHENOMENEX C18 (4.6 mm x 250 mm, 5 microns) chromatographic column, methanol-acetonitrile-water (15:15:10) as mobile phase. The wavelength for detection was 254 nm. RESULT: The peak of schizandrin appears on about 7.10 minutes. The standard curves of schizandrin were linear in the concentration range of 0.2-2.0 micrograms, r = 0.9996. The average recovery of schizandrin were 100.5% (RSD 2.84%). CONCLUSION: This method was found to be sensitive, quick and accurate for the measurement of schizandrin concentrations in Shengmaiyin.
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