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Title: [Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC]. Author: Jin WS, Tan YY, Chen YG, Wang Y. Journal: Zhongguo Zhong Yao Za Zhi; 2003 Jan; 28(1):49-51. PubMed ID: 15015267. Abstract: OBJECTIVE: To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata. METHOD: The separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1% HAC solution (gradient elution), the The detection wavelength being 250 nm. RESULT: The average recovery was respectively 101.7%, 100.7% and 101.7% (n = 3), RSD 0.43%, 0.82% and 1.50% (n = 3) for Puerarin, Daidzin and Daidzein. CONCLUSION: This method is suitable for the determination of Puerarin, Daidzin and Daidzein in Pueraria lobata and its preparation.[Abstract] [Full Text] [Related] [New Search]