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  • Title: In vivo detection of ribozyme cleavage products and RNA structure by use of terminal transferase-dependent PCR.
    Author: Chen HH, Castanotto D, LeBon J, Rossi JJ, Riggs AD.
    Journal: Methods Mol Biol; 2004; 252():109-24. PubMed ID: 15017045.
    Abstract:
    Terminal transferase-dependent PCR (TDPCR) can be used after reverse transcription to analyze RNA. This method (RT-TDPCR) is able to provide in vivo information at nucleotide-level resolution, and has been used for study of ribozymes, RNA size, RNA structure, and RNA-protein interactions. A detailed protocol of RT-TDPCR is presented here with examples of its use in detecting ribozyme cleavage intermediates in yeast and a RNA transcription start site in mammalian cells.
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